The AMR-NEQAS program was launched in May 2021 as an educational tool that enables participants to examine, monitor and improve their own laboratory methodologies and personnel performance by allowing comparison of results between the laboratories. With laboratories participating from the human, animal and food testing sector, the program aims to strengthen the NEQAS services across the One Health sector. The program is organized bi-annually and supported by the Fleming Fund project.In this round, six laboratories across the country participated in the scheme. The aim of this program is to monitor the quality of AST results produced by the participating laboratories, identify underperformers and accordingly design methods to enhance their performance in bacterial identification and AST. This report contains results from the second round of the NEQAS program June 2022. The program includes identification and performing AST on 03 test strains of a given organism. For each of the 03 test strains, participants are requested to report the expected target organism. One of the 03, is a blank sample, and no further testing is required. For the remaining two test strains of the target organism, results in relation to AST are requested. This round included identification, AST and serotyping of Salmonella spp. and Pseudomonas aeruginosa.