After the successfully completion of two years collaborative Surveillance between the Public Health Laboratory and the Department of Enteric Diseases, AFRIMS, Bangkok on Diarrhea Etiologic Agents in Stool Samples from Children Attending Jigme Dorji Wangchuk National Referral Hospital (JDWNRH), Thimphu, the collaborative surveillance on diarrhea etiologic agents has been extended to Eastern Regional Referral Hospital (Mongar), Central Regional Referral Hospital (Gelephu) and Phuntsholing General Hospital in July 2013 to further understand the epidemiology of diarrhea etiologic pathogens among the Bhutanese children for another two years.